Protein Microlute ™ P3 Protein Precipitation Plate
Biological samples commonly contain proteins
that interfere with downstream applications.
As the numbers of samples continue to
increase, the need for a high throughput
approach to remove these proteins is evident.
The P3 plate uses the CRASH method, in which
the protein is denatured with acetronitrile and
the flocculant filtered out, allowing 96 samples
to be handled at one time.
The Protein Precipitation Plate, P3, is based on
the industry standard Microlute™ format, but
without the chromatographic sorbent. Protein
‘crashes’ out of solution and precipitates
directly in each well when acetonitrile is added,
thus solving all common problems associated
with the CRASH technique of protein clean-up.
The novel dual frit, hydrophobically treated
matrix means that there is no ‘wetting out’ and
leakage of the sample through the plate before
the application of vacuum. P3 is now
commonly used as the protein precipitation
plate of choice in many major pharmaceutical
companies.